14 best lab thin layer chromatography tlc
Thin Layer Chromatography (TLC) is a widely used analytical technique in laboratories for separating and analyzing mixtures of compounds. Here's some information about lab TLC:
Principle of Separation: TLC operates on the principle of differential migration of components in a mixture over a thin layer of adsorbent material.It's similar to column chromatography but is faster and more suitable for qualitative analysis.
Components:
- Stationary Phase: The stationary phase is a thin layer of an adsorbent material, often silica gel or alumina, coated onto a flat, inert support like glass or plastic.
- Mobile Phase: The mobile phase is a solvent or a mixture of solvents that migrates up the TLC plate by capillary action. It carries the sample components with it.
Sample Application: A small amount of the mixture to be analyzed is spotted onto the TLC plate near the bottom.
Development: The plate is then placed in a developing chamber containing a suitable solvent system. The solvent moves up the plate, carrying the components of the mixture with it.
Separation: As the solvent migrates up the plate, different components of the mixture will interact differently with the stationary phase. This results in the separation of the components into distinct spots or bands on the TLC plate.
Visualization: After the TLC plate has been developed, it needs to be visualized. This can be done through methods like UV light (for compounds that absorb UV light), staining reagents, or by using a spray reagent that reacts with specific functional groups.
Rf Value: The Retention Factor (Rf) value is a ratio used to identify and compare compounds. It's calculated as the distance traveled by the compound divided by the distance traveled by the solvent front.
Applications:
- Qualitative Analysis: TLC is often used for the identification of compounds in a mixture. It's quick and provides visual results.
- Purity Checking: It can be used to assess the purity of a compound by comparing the number of spots with the expected number.
- Reaction Monitoring: TLC is used to monitor the progress of chemical reactions.
Lab Instruments: In a laboratory setting, equipment for TLC includes TLC plates, a developing chamber, capillary tubes or pipettes for sample application, and visualization tools.
Safety: As with any laboratory technique, safety precautions should be followed, including proper handling of chemicals and adherence to laboratory protocols.
TLC is a versatile and widely used technique in various scientific disciplines including chemistry, biochemistry, and pharmacology. It's a valuable tool for both qualitative and quantitative analysis.
Below you can find our editor's choice of the best lab thin layer chromatography tlc on the market
TLC Plates Aluminium Backed Silica Gel 60 F254 2.5 x 7.5cm （80/Box）
LICHEN COTTAGE
Product description
Product features：
1. The thin-layer chromatography silica gel plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder. The surface of the plate is pure white, flat and even, fine, and no peeling. The base plate of this product is aluminum foil.
2. The main component is SiO2·nH2O, which has stable chemical property and doesn’t react with other acid or alkali except for strong alkali and hydrofluoric acid. The thin-layer chromatography silica gel plates can be used directly for qualitative and quantitative analysis of various types of organic substances.
Special notes: Aluminum foil material is used on the back of aluminum foil chromatography silica gel plate, which will inevitably generate aluminum rust. However, you don't need to worry, the product has no problem and it will not affect the normal use, as long as the front of aluminum foil silica gel plate is used normally!
【We are dedicated in providing the best products and services to customers. If you encounter any problem, please don’t hesitate to contact us. We will reply your e-mail in no more than 8 hours since we receive it, and we will help you solve the problem as soon as possible】
- 【Easily Cut】 ：Silica gel GF254， It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254. The aluminum back can be easily cut open with scissors.
- 【Good separation effect】: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute.
- 【High sensitivity】: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis.
- 【We are dedicated in providing the best products and services to customers. If you encounter any problem, please don’t hesitate to contact us. We will reply your e-mail in no more than 8 hours since we receive it, and we will help you solve the problem as soon as possible】
StonyLab Glass Backed TLC Classical Silica Gel Plate, 2.5 x 7.5 cm Thin Layer Chromatography Analytical Plate with Silica Gel, Pack of 80
StonyLab Scientific
Product description
Why Choose StonyLab?
1. The StonyLab means high-quality products for reasonable prices.
2. We boast excellent customer service with a high satisfaction rating, with our team of representatives ready to assist you Monday to Friday, from 9:30AM to 5:30PM Eastern Time.
3. We pack and ship your orders directly from our US-based warehouse within 24 business hours, and tracking information will be provided to you as soon as it's available.
Description
Pack of 80, Glass backed TLC silica gel plate
Size: 2.5 x 7.5 cm
Layer thickness: 200 µm ± 30 µm
PH: 6.2 - 6.8
Made with unmodified silica gel and polymeric binder for adherent surface. Featured with high chemical resistance, high efficiency and high fluorescence
StonyLab ensures high quality and premium customer service
- Pack of 80, Glass backed TLC silica gel plate
- Size: 2.5 x 7.5 cm
- Layer thickness: 200 µm ± 30 µm
- PH: 6.2 - 6.8
- Made with unmodified silica gel and polymeric binder for adherent surface; Featured with high chemical resistance, high efficiency and high fluorescence
Laboratory Glass Thin Silicone Plate Cutter Chromatography Plate TLC Cutter Cutting Machine
top-tool
Product description
Laboratory Glass Thin Silicone Plate Cutter Chromatography Plate TLC Cutter Cutting Machine
Applications:
The instrument is designed to cut thin sheets and is used to cut thin-layer chromatography plates of the specified size
Features:
Machine size: 280*240*65mm
Suitable for precise cutting of thin plates
It can cut thin plates up to 3mm-5mm thick, which is convenient and quick
Easy to use, you can read the required size directly on the scale
Precise cutting, effectively avoiding the waste caused by cutting
Fast shipping with DHL/FEDEX/UPS/EMS or other express shipping service!
- The instrument is designed to cut thin sheets and is used to cut thin-layer chromatography plates of the specified size
- Suitable for precise cutting of thin plates
- It can cut thin plates up to 3mm-5mm thick, which is convenient and quick
- Easy to use, you can read the required size directly on the scale
- Precise cutting, effectively avoiding the waste caused by cutting
EMD Millipore 1.12537.0001 UV Lamp for Thin-Layer Chromatography, 254 nm
EMD Millipore Corporation
Product description
Our TLC accessories allow professionals to work more quickly, more efficiently, and more reliably. Thanks to our broad assortment, users will always find what they need.
Glass TLC Plate Cutter up to 20cm Wide Thin Layer Chromatography General Glass Cutter AAdvance Instruments (Replacement Scribbler Blade)
Shijia Biomedical Tech. Co. (Changzhou)
Product description
Replacement Scribbler Blade ONLY, for Shijia Biomedical Tech 20cm Glass TLC Plate Cutter
- Replacement Scribbler Blade ONLY, for Shijia Biomedical Tech 20cm Glass TLC Plate Cutter
Wilmad LG-4590-100 Chromatography TLC Sprayer Complete, 125 mL Capacity
SP Scienceware
Product description
When using thin layer or paper chromatography (TLC), these borosilicate glass atomizers are used for spraying chromogenic reagents. Complete unit consists of spray head, flask, and LG-1004 clamp. The air inlet tube has an opening to release pressure and stop spraying. All joints are Standard Taper 24/40. Scientists rely on the expertise and artistic skills of Wilmad-LabGlass' engineers and glass specialists to manufacture NMR and EPR consumables and laboratory glassware of the highest quality. Used daily in academia, healthcare and chemical, petroleum and other industrial applications, Wilmad offers 7000+ items as part of their standard catalog as well as custom fabrication for individual glassware, glass repair services and OEM glass parts.
Classic Glass Preparative TLC PLC Plates Silica Gel 60 F254 1 or 2 mm Layer Thickness (1000 or 2000 μm/microns) 20 x 20 cm Size 8 or 6 Plates/Box AAdvance Instruments (1 mm, 8)
Yantai Dexin Biotech
Product description
Classic Preparative TLC Plates. Silica Gel 60 F254 type. 20x20cm plate size, 1mm (1000μm) silica layer thickness. pH 6.2-6.8, particle size 10-40 μm, pore size 60 Å. 8 plates per box. Glass backed.
A test run separating dimethyl maleate and dimethyl fumerate has been performed and shown in photos.
50mg of each compound (total mass 100mg), dissolved in a small amount mixture of hexane, acetone and dichloromethane was spotted onto the plate. The plate was eluted twice with hexane:acetone=15:1, giving a very good separation. While this is expected, this also indicated that depending on how far away each component band is on TLC, one can load material amounts of 100mg and more onto the plate since in the example the plates have not quite reached full capacity.
The preparative TLC method is an alternative to preparative HPLC. HPLC methods can be more expensive and when handled improperly, contamination can occur. Prep TLC is especially suitable for applications when there is relatively a small amount of sample, and purity requirements are high, and when at the same time the researcher is looking for a quick, convenient and economic way of purification.
---------------------------------
Large capillary tubes for sample spotting: www.amazon.com/dp/B07LDB95CZ
- Classic Preparative TLC Plates. Silica Gel 60 F254 type. 20x20cm plate size, 1mm (1000μm) silica layer thickness. pH 6.2-6.8, particle size 10-40 μm, pore size 60 Å. 8 plates per box. Glass backed.
- A test run separating dimethyl maleate and dimethyl fumerate has been performed and shown in photos.
- 50mg of each compound (total mass 100mg), dissolved in a small amount mixture of hexane, acetone and dichloromethane was spotted onto the plate. The plate was eluted twice with hexane:acetone=15:1, giving a very good separation. While this is expected, this also indicated that depending on how far away each component band is on TLC, one can load material amounts of 100mg and more onto the plate since in the example the plates have not quite reached full capacity.
- The preparative TLC method is an alternative to preparative HPLC. HPLC methods can be more expensive and when handled improperly, contamination can occur. Prep TLC is especially suitable for applications when there is relatively a small amount of sample, and purity requirements are high, and when at the same time the researcher is looking for a quick, convenient and economic way of purification.
- Return Policy: Have peace of mind in shopping. Whether your purchase of this item is fulfilled by Amazon, or fulfilled by seller, you may return it within 30 days of receipt, as per Amazon policy.
Kimble 422530-0025 Chromatography TLC Reagent Sprayer with Screw Thread Ground Joints, 19/22 Standard Taper Joint, 25mL Capacity
Kimble
Product description
The connection between the sprayer head and the flask is made with a special screw thread ground joint, connecting cap and loosening ring. If the joint ever "freezes" from reagent crystallization or residue formation, simply unscrew the connecting cap back onto the unique loosening ring and the joint will easily come apart. These sprayers will operate on as little as 1 psi (0.07 kg/cm²) air pressure with optimal results at 3-5 psi (0.21-0.35 kg/cm²). The connecting cap, loosening ring and o-ring should be removed before autoclaving.
- Package Dimensions: 27.94 cms (L) x 19.304 cms (W) x 10.414 cms (H)
- Product Type: Lab Supply
- Package Quantity: 1
- Country Of Origin: United States
Wilmad-Lab Glass LG-4590-110 TLC Sprayer Flask, 125 mL Capacity
SP Industries, Inc.
Product description
For spraying chromogenic reagents when using thin layer or paper chromatography. Complete unit consists of the spray head, flask and lg-1004 clamp. Joints are 24/40. The air Inlet tube has an opening to release pressure and stop spraying.
805021, TLC Plate Polygram 60A Polyester Sheet Silica 0.2mm Layer, UV254 4cm x 8cm, Pack of 50
Macherey-Nagel
Product description
POLYGRAM SIL G UV254, 4x8 cm -pack of 25
- Polygram Sil G Uv254, 4X8 cm, Pack Of 25
User questions & answers
| Question: | Is it good for chromatography use |
| Answer: | Yes (thin layer chromatography). |
Product features
POLYGRAM SIL G/UV254
Macherey-Nagel, 805021, POLYGRAM SIL G/UV254, unmodified standard silica layers on polyester (plastic) for TLC, layer thickness 0.2 mm, size 4 x 8 cm, pack of 50.
Lab Thin Layer Chromatography TLC Plates P-1 Type Developing Tanks,Double Slot,Length × Height 100 × 200mm
Adamas-Beta
Product description
Lab Thin Layer Chromatography TLC Plates P-1 Type Developing Tanks,Double slot,Length × height 100 × 200mm
- High quality borosilicate glass materials, chemically stable, no chemical reaction with reagents
- The bottom of the P-cylinder cylinder is flat, and it is formed by flat glass double right angle, which has good flatness and sealing
- Beautiful shape, correct specifications, flat bottom, tight cover, easy to clean
- Convenient, fast, good effect, high sensitivity
- Perfect For Research Or Teaching Tasks
EMD Millipore 1.07749.1000 Silica Gel 60 PF254 Sorbent for Preparative Layer Chromatography, 1kg Plastic Bottle
EMD Millipore
Product description
Standardized sorbents ensure reliable results every time. EMD Millipore's broad spectrum meets all kinds of needs. In TLC, silica gel 60 sorbent is considered the most versatile. Our assortment of silica gel 60 features different grades with particle sizes ranging from 5-40 µm: silica with a gypsum binder, silica with no foreign binder, and silica gel with a fluorescence indicator. We also offer high-quality aluminum oxide, cellulose microcrystalline, and kieselgur (also known as diatomaceous earth).
- Package Dimensions :
- Item model number :
- Date First Available :
- Manufacturer :
- ASIN :
Adamas-Beta P-1 Chromatography TLC Developing Chamber Tank Holds 2 Plates Lid Included AAdvance Instruments, Length×Height 100×100mm/3.94×3.94in
Adamas-Beta
Product description
Specifications
Pack: 1
Material: borosilicate glass
L×H: 100×100mm/3.94×3.90in
Characteristics
Made of good quality borosilicate glass, durable.
Less solvent consumption,Repeatable solvent vapor pre-adsorption.
Stable physicochemical properties, no chemical reaction with solvents.
TLC developing tank for plates up to 100mm by 100mm. Fits 2 plates with 2 separate solvent bays.
It is widely used in environmental protection, pharmaceutical and biological inspection, industrial product quality inspection, etc.
- Made of good quality borosilicate glass, durable.
- TLC developing tank for plates up to 100mm by 100mm.Fits 2 plates with 2 separate solvent bays.
- Beautiful shape, tight cover, good effect, high sensitivity, easy to clean.
- Stable physicochemical properties, no chemical reaction with solvents.
- It is widely used in environmental protection, pharmaceutical and biological inspection, industrial product quality inspection, etc.
Eisco Labs Chromatography Filter Paper Sheet 25cm x 25cm (9.84" x 9.84") Pack of 100
EISCO
Product description
Eisco Labs Chromatography Filter Paper Sheet 25cm x 25cm -(9.84" x 9.84") Pack of 100 , Chromatography is a method used by scientists for separating organic and inorganic compounds so that they can be analyzed and studied
- Chromatography is a method used by scientists for separating organic and inorganic compounds so that they can be analyzed and studied
- Sheets are 25cm x 25cm ( 9.84" x 9.84" )
- Pack of 100
User questions & answers
| Question: | Is this paper stiff enough to roll into a cylinder and use for ascending chromatography |
| Answer: | Yes I believe it can be rolled into a cylinder. I do not use it for ascending chromatography but I don’t see why it could not be used for that purpose. |
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